6533b834fe1ef96bd129d544

RESEARCH PRODUCT

Optimal design of incoherent tunable-frequency structured illumination microscope scheme

Chrysanthe PrezaAna DoblasJorge Sola-pikabeaManuel Martínez-corralGenaro Saavedra

subject

Optimal designScheme (programming language)MicroscopeOptical sectioningbusiness.industryComputer scienceResolution (electron density)Visibility (geometry)ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONStructured illumination microscopyField of viewSample (graphics)law.inventionOpticslawbusinesscomputercomputer.programming_language

description

Structured illumination microscopy (SIM) improves resolution and optical sectioning capability compared to conventional widefield techniques. The main idea of this method is the illumination of the sample with a structured pattern of fixed spatial modulation frequency. Previously, a Fresnel biprism has been implemented in a structured illumination (SI) device providing tunable-frequency sinusoidal patterns. However, the use of this SI system introduces a tradeoff between the visibility and field of view of the illumination fringes. In this contribution, we analyze theoretically this tradeoff and propose the optimal design for the Fresnel biprism-based SIM system.

yearjournalcountryeditionlanguage
2018-04-012018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018)
https://doi.org/10.1109/isbi.2018.8363613