6533b86dfe1ef96bd12c9f75

RESEARCH PRODUCT

FIMic: design for ultimate 3D-integral microscopy of in-vivo biological samples

J.c. BarreiroGenaro SaavedraManuel Martínez-corralJorge Garcia-sucerquiaGabriele ScrofaniJorge Sola-pikabeaEmilio Sánchez-ortigaAnabel Llavador

subject

Materials scienceMicroscope02 engineering and technology01 natural sciencesArticlelaw.invention010309 opticsBiological specimenOpticsOptical microscopelaw0103 physical sciencesMicroscopyDepth of fieldImage resolutionMicrolensbusiness.industryResolution (electron density)Òptica021001 nanoscience & nanotechnologyAtomic and Molecular Physics and OpticsMicroscòpia0210 nano-technologybusinessImatges tridimensionals en biologiaBiotechnology

description

In this work, Fourier integral microscope (FIMic), an ultimate design of 3D-integral microscopy, is presented. By placing a multiplexing microlens array at the aperture stop of the microscope objective of the host microscope, FIMic shows extended depth of field and enhanced lateral resolution in comparison with regular integral microscopy. As FIMic directly produces a set of orthographic views of the 3D-micrometer-sized sample, it is suitable for real-time imaging. Following regular integral-imaging reconstruction algorithms, a 2.75-fold enhanced depth of field and [Formula: see text]-time better spatial resolution in comparison with conventional integral microscopy is reported. Our claims are supported by theoretical analysis and experimental images of a resolution test target, cotton fibers, and in-vivo 3D-imaging of biological specimens.

https://doi.org/10.1364/boe.9.000335