Search results for "Microscòpia"
showing 10 items of 32 documents
Stable and simple quantitative phase-contrast imaging by Fresnel biprism
2018
Digital holographic (DH) microscopy has grown into a powerful nondestructive technique for the real-time study of living cells including dynamic membrane changes and cell fluctuations in nanometer and sub-nanometer scales. The conventional DH microscopy configurations require a separately generated coherent reference wave that results in a low phase stability and a necessity to precisely adjust the intensity ratio between two overlapping beams. In this work, we present a compact, simple, and very stable common-path DH microscope, employing a self-referencing configuration. The microscope is implemented by a diode laser as the source and a Fresnel biprism for splitting and recombining the be…
View images with unprecedented resolution in integral microscopy
2018
Integral microscopy is a novel technique that allows the simultaneous capture of multiple perspective images of microscopic samples. This feature is achieved at the cost of a significant reduction of the spatial resolution. In fact, it is assumed that in the best cases the resolution is reduced by a factor that is not smaller than ten, what poses a hard drawback to the utility of the technique. However, to the best of our knowledge, this resolution limitation has never been researched rigorously. For this reason, the aim of this paper is to explore the real limitations in resolution of integral microscopy and to obtain optically, without the need of any image-processing algorithm, perspecti…
Creixement del MgCdO: un nou aliatge ternari d'òxids II-VI
2017
Els nous reptes tecnològics impulsen el desenvolupament de nous materials amb les propietats físiques adients per satisfer les necessitats de cada aplicació. Així, el desenvolupament de nous materials optoelectrònics ha rebut una gran atenció de la comunitat científica els últims anys. Més concretament, els compostos II-VI com els òxids de Zn, Cd i Mg o els aliatges ternaris ZnMgO i ZnCdO han sigut i estan sent àmpliament estudiats degut a les seues propietats físiques que els doten d'un gran potencial per al seu ús en dispositius optoelectrònics com díodes emissors de llum (LED per les sigles en anglès de Light-Emitting Diode) o sensors de radiació ultraviolada [Nieda2016, Wang2010, Mohant…
Area-Based Depth Estimation for Monochromatic Feature-Sparse Orthographic Capture
2018
With the rapid development of light field technology, depth estimation has been highlighted as one of the critical problems in the field, and a number of approaches have been proposed to extract the depth of the scene. However, depth estimation by stereo matching becomes difficult and unreliable when the captured images lack both color and feature information. In this paper, we propose a scheme that extracts robust depth from monochromatic, feature-sparse scenes recorded in orthographic sub-aperture images. Unlike approaches which rely on the rich color and texture information across the sub-aperture views, our approach is based on depth from focus techniques. First, we superimpose shifted …
Microscopic study of He2-SF6 trimers
2003
The He2-SF6 trimers, in their different He isotopic combinations, are studied in the framework of both the correlated Jastrow approach and the correlated hyperspherical harmonics (CHH) expansion method. The energetics and structure of the He-SF6 dimers are analyzed, and the existence of a characteristic rotational band in the excitation spectrum is discussed, as well as the isotopic differences. The binding energies and the spatial properties of the trimers, in their ground and lowest lying excited states, obtained by the Jastrow ansatz are in excellent agreement with the results of the converged CHH expansion. The introduction of the He-He correlation makes all trimers bound by largely sup…
Digital holographic microscopy as a screening technology for diabetes
2019
Label-free quantitative phase imaging (QPI) is the hallmark of digital holographic microscopy (DHM). One of the most interesting medical applications of QPI-DHM is that it can be used to analyze illnesses in which the refractive index or/and the morphology of cells/tissues are distorted, from the acquisition of a single image. In this contribution, we obtain the phase maps of red blood cells (RBCs) samples of patients suffering from diabetes mellitus type 1 (DM1) by using a DHM. Our experimental results show that the measured phase values are significantly different between control non-diabetic and diabetic patients. The high correlation coefficient between the phase and the glycated hemogl…
Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy.
2019
A method of determining unknown phase-shifts between elementary images in two-dimensional Structured Illumination Microscopy (2D-SIM) is presented. The proposed method is based on the comparison of the peak intensity of spectral components. These components correspond to the inherent structured illumination spectral content and the residual compo- nent that appears from wrongly estimated phase-shifts. The estimation of the phase-shifts is carried out by finding the absolute maximum of a function defined as the normalized peak intensity difference in the Fourier domain. This task is performed by an optimization method providing a fast estimation of the phase-shift. The algorithm stability an…
Phase-shifting by means of an electronically tunable lens: quantitative phase imaging of biological specimens with digital holographic microscopy
2016
The use of an electronically tunable lens (ETL) to produce controlled phase shifts in interferometric arrangements is shown. The performance of the ETL as a phase-shifting device is experimentally validated in phase-shifting digital holographic microscopy. Quantitative phase maps of a section of the thorax of a Drosophila melanogaster fly and of human red blood cells have been obtained using our proposal. The experimental results validate the possibility of using the ETL as a reliable phase-shifter device.
Optical sectioning microscopy through single-shot Lightfield protocol
2020
Optical sectioning microscopy is usually performed by means of a scanning, multi-shot procedure in combination with non-uniform illumination. In this paper, we change the paradigm and report a method that is based in the light field concept, and that provides optical sectioning for 3D microscopy images after a single-shot capture. To do this we fi rst capture multiple orthographic perspectives of the sample by means of Fourier-domain integral microscopy (FiMic). The second stage of our protocol is the application of a novel refocusing algorithm that is able to produce optical sectioning in real time, and with no resolution worsening, in the case of sparse f luorescent samples.We provide the…
The Lightfield Microscope Eyepiece
2021
Lightfield microscopy has raised growing interest in the last few years. Its ability to get three-dimensional information about the sample in a single shot makes it suitable for many applications in which time resolution is fundamental. In this paper we present a novel device, which is capable of converting any conventional microscope into a lightfield microscope. Based on the Fourier integral microscope concept, we designed the lightfield microscope eyepiece. This is coupled to the eyepiece port, to let the user exploit all the host microscope’s components (objective turret, illumination systems, translation stage, etc.) and get a 3D reconstruction of the sample. After the optical design, …