6533b855fe1ef96bd12b0931

RESEARCH PRODUCT

The Lightfield Microscope Eyepiece

Gabriele ScrofaniNicolò IncardonaManuel Martínez-corralÁNgel TolosaGenaro Saavedra

subject

FiMicMicroscopeComputer scienceComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONTP1-1185Translation (geometry)BiochemistryAnalytical Chemistrylaw.inventionEyepieceOpticslawlightfield eyepieceMicroscopyplenoptic eyepieceElectrical and Electronic EngineeringInstrumentationLightingFourier integral microscopeMicroscopylightfield microscopybusiness.industryCommunicationChemical technology3D reconstructionSingle shotTime resolutionSample (graphics)Atomic and Molecular Physics and OpticsÒptica Aparells i instrumentsMicroscòpia3D microscopybusiness

description

Lightfield microscopy has raised growing interest in the last few years. Its ability to get three-dimensional information about the sample in a single shot makes it suitable for many applications in which time resolution is fundamental. In this paper we present a novel device, which is capable of converting any conventional microscope into a lightfield microscope. Based on the Fourier integral microscope concept, we designed the lightfield microscope eyepiece. This is coupled to the eyepiece port, to let the user exploit all the host microscope’s components (objective turret, illumination systems, translation stage, etc.) and get a 3D reconstruction of the sample. After the optical design, a proof-of-concept device was built with off-the-shelf optomechanical components. Here, its optical performances are demonstrated, which show good matching with the theoretical ones. Then, the pictures of different samples taken with the lightfield eyepiece are shown, along with the corresponding reconstructions. We demonstrated the functioning of the lightfield eyepiece and lay the foundation for the development of a commercial device that works with any microscope.

yearjournalcountryeditionlanguage
2021-10-01Sensors
https://doi.org/10.3390/s21196619