6533b82dfe1ef96bd1291543

RESEARCH PRODUCT

Scalp microbiota alterations in children with pediculosis.

Raquel Vizzotto De MenezesJuciliane HaidamakDébora Do Rocio KlisiowiczVânia Aparecida VicenteAmanda Albino BissonBruna Jacomel Favoreto De Souza LimaRenata R. GomesAmanda Santos TaleviGermana Davila Dos SantosM. A. ValeroValéria Mendes Soares

subject

0301 basic medicineMicrobiology (medical)MaleTetracycline030106 microbiologyPediculosisBiologyMicrobiologyDNA RibosomalGroup BMicrobiologyAgar plate03 medical and health sciencesStaphylococcus epidermidisGeneticsmedicineAnimalsHumansChildMolecular BiologyRibosomal DNAEcology Evolution Behavior and SystematicsScalpMicrobiotaPediculusLice Infestationsbiology.organism_classificationmedicine.diseaseStaphylococcus capitis030104 developmental biologyInfectious DiseasesFemaleBacteriamedicine.drug

description

Pediculosis is a disease caused by the insect Pediculus humanus capitis that mainly occurs in childhood. A comparative study was carried out evaluating groups of schoolchildren with (group A) and without pediculosis (group B) to analyse the characteristics of the scalp microbiota. Samples were collected by swab using Stuart transport medium and incubate in Sabouraud dextrose agar with tetracycline to analyse the fungal microbiota and in blood agar to assess the bacterial microbiota. The isolates identity was confirmed by sequencing of the 16S and 18S regions of the ribosomal DNA gene for bacteria and fungi, respectively. The analysis of the 186 isolates led to the identification of 35 bacteria and 40 fungi in group A and 47 bacteria and 64 fungi in group B. The results indicate differences in bacterial and fungal species in the groups analysed. In the observed bacterial microbiota, Staphylococcus capitis occurred more frequently than Staphylococcus epidermidis in group A vs B. Among fungal isolates, Debaryomyces sp. was more frequent in group B vs A. Our findings showed scalp microbiota alterations in children with pediculosis, meriting future studies to analyse the relationship between these agents and their impact on human health.

yearjournalcountryeditionlanguage
2019-01-12Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
10.1016/j.meegid.2019.05.016https://pubmed.ncbi.nlm.nih.gov/31121305