6533b82dfe1ef96bd12915e4

RESEARCH PRODUCT

Cell population analysis of the adult murine subependymal neurogenic lineage by flow cytometry

German BelenguerJosé Manuel Morante-redolatAna Domingo-muelasPere Duart-abadiaIsabel Fariñas

subject

MaleScience (General)Lineage (genetic)CellPopulationCell Culture TechniquesSingle CellBiologyGeneral Biochemistry Genetics and Molecular BiologyCell LineFlow cytometryTranscriptomeMiceQ1-390Neural Stem CellsEpendymaProtocolmedicineSubependymal zoneAnimalsFlow Cytometry/Mass Cytometryeducationeducation.field_of_studyGeneral Immunology and Microbiologymedicine.diagnostic_testGene Expression ProfilingStem CellsGeneral NeuroscienceCell BiologyFlow CytometryNeural stem cellCell biologyMice Inbred C57BLmedicine.anatomical_structureFemaleSingle-Cell AnalysisStem cellTranscriptomeNeuroscience

description

Summary This protocol provides a flow-cytometry-based procedure to classify and isolate all cells of the adult rodent subependymal zone (SEZ) neurogenic lineage, without the need for reporter mice, into different cell populations, including three neural stem cell (NSC) fractions with molecular signatures that are coherent with single-cell transcriptomics. Additionally, their cycling behavior can be assessed by means of 5-ethynyl-2′-deoxyuridine (EdU) incorporation. Our method allows the isolation of different NSC fractions and the functional assay of their cycling heterogeneity and quiescence-activation transitions. For complete details on the use, execution, and outcomes of this protocol, please refer to Belenguer et al. (2021).

yearjournalcountryeditionlanguage
2021-06-01STAR Protocols
https://doi.org/10.1016/j.xpro.2021.100425