6533b82dfe1ef96bd1291d8d
RESEARCH PRODUCT
Speckle random coding for 2D super resolving fluorescent microscopic imaging.
Zeev ZalevskyDror FixlerAryeh WeissMordechai DeutschJavier Garciasubject
DiffractionPhysicsMicroscopebusiness.industryResolution (electron density)General Physics and AstronomySpeckle noiseCell BiologyNumerical aperturelaw.inventionSpeckle patternOpticsStructural BiologylawElectronic speckle pattern interferometryGeneral Materials ScienceProjection (set theory)businessdescription
In this manuscript we present a novel super resolving approach based upon projection of a random speckle pattern onto samples observed through a microscope. The projection of the speckle pattern is created by coherent illumination of the inspected pattern through a diffuser. Due to local interference of the coherent wave front with itself, a random speckle pattern is superimposed on the sample. This speckle pattern can be scanned over the object. A super-resolved image can be extracted from a temporal sequence of images by appropriate digital processing of the image stream. The resulting resolution is significantly higher than the diffraction limitation of the microscope objective. The new super-resolution method is demonstrated by application to fluorescence of biological samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-09-02 | Micron (Oxford, England : 1993) |