A new method for the cytochemical demonstration ofp-diphenol: O2 oxidoreductase (laccase)

6533b82efe1ef96bd129293c

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A new method for the cytochemical demonstration ofp-diphenol: O2 oxidoreductase (laccase)

subject

Pharmacology Laccase chemistry.chemical_classification Histocytochemistry Chemistry Aspergillus fumigatus Cell Biology Molecular biology Neurospora Cellular and Molecular Neuroscience Aspergillus Oxidoreductase Methods Molecular Medicine Mitosporic Fungi Molecular Biology Catechol Oxidase

description

Nachweis des Enzymsp-Diphenol: O2 oxidoreductase (Laccase) in den Zellen der PilzeAspergillus fumigatus, Aureobasidium pullulans undNeurospora sitophila durch einen Azofarbstoff, der mittels Kupplung des enzymatisch gebildetenp-Chinons mitBesthorn's Hydrazon(3-Methyl-benzthiazolon(2)-hydrazon-hydrochlorid) entsteht. Als Substrat wird Hydrochinon verwendet. Der Farbstoff wird in runden, rotbraunen Granula abgelagert. Kontrollreaktionen bestatigen die Spezifitat der Reaktion.

year	journal	country	edition	language
1971-05-15	Experientia

https://doi.org/10.1007/bf02147631

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