Comparison of Two Solid-Phase Extraction (SPE) Methods for the Identification and Quantification of Porcine Retinal Protein Markers by LC-MS/MS

6533b82efe1ef96bd1292a64

RESEARCH PRODUCT

Comparison of Two Solid-Phase Extraction (SPE) Methods for the Identification and Quantification of Porcine Retinal Protein Markers by LC-MS/MS

Sebastian Funke Franz H. Grus Jana Treml Norbert Pfeiffer Carsten Schmelter Anja Beschnitt Natarajan Perumal Caroline Manicam

subject

0301 basic medicine Swine General Mathematics sample clean-up Proteomics Mass spectrometry 01 natural sciences Catalysis Retina Article lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound glaucoma animal model ZIPTIP® C18 pipette tips Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Trifluoroacetic acid Animals Sample preparation Solid phase extraction Physical and Theoretical Chemistry Eye Proteins lcsh:QH301-705.5 Molecular Biology Spectroscopy mass spectrometry ZIPTIP<sup>®</sup> C18 pipette tips Reproducibility Chromatography Chemistry Applied Mathematics 010401 analytical chemistry Organic Chemistry Solid Phase Extraction Extraction (chemistry)Pipette biomarkers Glaucoma General Medicine 0104 chemical sciences Computer Science Applications 030104 developmental biology lcsh:Biology (General)lcsh:QD1-999 SOLAμTM HRP SPE spin plates Peptides Chromatography Liquid

description

Proper sample preparation protocols represent a critical step for liquid chromatography-mass spectrometry (LC-MS)-based proteomic study designs and influence the speed, performance and automation of high-throughput data acquisition. The main objective of this study was to compare two commercial solid-phase extraction (SPE)-based sample preparation protocols (comprising SOLA&micro

year	journal	country	edition	language
2018-12-03	International Journal of Molecular Sciences

10.3390/ijms19123847 http://dx.doi.org/10.3390/ijms19123847

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