6533b82efe1ef96bd1293d1c
RESEARCH PRODUCT
Identification of an LPS-Induced Chemo-Attractive Peptide from Ciona robusta
Melchiorre CervelloAntonino LauriaValeria LongoAnnamaria MartoranaGiuseppa AugelloAntonina AzzolinaAlessandra LongoPaolo Colombosubject
In silicoPharmaceutical ScienceMotilitychemoattractive peptide03 medical and health sciencesAdapter molecule crk0302 clinical medicineWestern blotDrug DiscoverymedicineNF-kBCiona robustaPharmacology Toxicology and Pharmaceutics (miscellaneous)<i>Ciona robusta</i>lcsh:QH301-705.5030304 developmental biology0303 health sciencesmedicine.diagnostic_testChemistryfungiIn vitro3D modellingCell biologyBlotlcsh:Biology (General)Cell cultureinflammation030220 oncology & carcinogenesisSignal transductionCiona robusta; inflammation; chemoattractive peptide; NF-kB; 3D modellingdescription
Background: Previously published work has demonstrated that the LPS injection of Ciona robusta leads to the overexpression of a truncated form of an immune-related mRNA (C8short) by means of Ciona robusta (CR) alternative polyadenylation (APA) (CR-APA). Methods: The 3D structure of the C8short-derived Ciona robusta chemo-attractive peptide (CrCP) was evaluated by homology modeling. The biological activity of the CrCP was studied in vitro using a primary human dermal cell line (HuDe). Real-Time PCR was used to investigate the expression levels of genes involved in cell motility. NF-&kappa
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-04-01 | Marine Drugs |