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RESEARCH PRODUCT
Design and physicochemical characterization of poly(amidoamine) nanoparticles and the toxicological evaluation in human endothelial cells: applications to peptide delivery to the brain
Johannes F.j. EngbersenG.m.j.p.c. CouéR.e. UngerChristian FreeseKarin E. PicklFrank SinnerCharles James Kirkpatricksubject
Materials scienceAmidoamineeducationBiomedical EngineeringBiophysicsNanoparticleBioengineeringPeptideUmbilical veinBiomaterialschemistry.chemical_compoundMETIS-302365Human Umbilical Vein Endothelial CellsPolyaminesIR-90176HumansCytotoxicityCells Culturedchemistry.chemical_classificationDrug CarriersIntracellular proteinBrainEndothelial CellsPoly(amidoamine)chemistryBiochemistryDrug deliveryMicrovesselsBiophysicsNanoparticlesOligopeptidesdescription
In this study, we investigated nanoparticles formulated by self-assembly of a biodegradable poly(amidoamine) (PAA) and a fluorescently labeled peptide, in their capacity to internalize in endothelial cells and deliver the peptide, with possible applications for brain drug delivery. The nanoparticles were characterized in terms of size, surface charge, and loading efficiency, and were applied on human cerebral microvascular endothelial cells (hCMEC/D3) and human umbilical vein endothelial cells (Huvec) cells. Cell-internalization and cytotoxicity experiments showed that the PAA-based nanocomplexes were essentially nontoxic, and the peptide was successfully internalized into cells. The results indicate that these PAAs have an excellent property as nontoxic carriers for intracellular protein and peptide delivery, and provide opportunities for novel applications in the delivery of peptides to endothelial cells of the brain.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-01-01 | Journal of biomaterials science : polymer edition |