6533b82ffe1ef96bd129477a
RESEARCH PRODUCT
Effect of cholesterol on the physical properties of pulmonary surfactant films: Atomic force measurements study
Matthias AmreinZoya LeonenkoEric FinotVladislav Vassilievsubject
Models Molecular12-DipalmitoylphosphatidylcholineSurface PropertiesFunctional failureLipid BilayersAnalytical chemistryMicroscopy Atomic ForceSurface tensionchemistry.chemical_compoundRigidity (electromagnetism)Pulmonary surfactantAnimalsSurface TensionInstrumentationAtomic force microscopyCholesterolPulmonary SurfactantsAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsCholesterol[ PHYS.PHYS.PHYS-AO-PH ] Physics [physics]/Physics [physics]/Atmospheric and Oceanic Physics [physics.ao-ph]chemistryMechanical stabilityPhosphatidylcholinesBiophysicsCattleAdhesivedescription
International audience; Atomic force measurements were performed on supported pulmonary surfactant (PS) films to address the effect of cholesterol on the physical properties of lung surfactant films. We recently found that cholesterol in excess of a physiological proportion abolishes surfactant function, and is the reason that surfactant fails to lower the surface tension upon compression. In this study, we investigated how the loss of mechanical stability observed earlier is related to the local mechanical properties of the film by local force measurements. The presence of 20% of cholesterol in bovine lipid extract surfactant (BLES) resulted in a decrease of the observed adhesive interaction, and an increase in rigidity of the film. We discuss the implication the increased rigidity might have on the functional failure of PS.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-01-01 | Ultramicroscopy |