6533b82ffe1ef96bd1294874
RESEARCH PRODUCT
Extended analysis of a genome-wide association study in primary sclerosing cholangitis detects multiple novel risk loci
Tom H. KarlsenTom H. KarlsenDavid EllinghausCyriel Y. PonsioenErik SchrumpfErik SchrumpfAndreas TeufelTobias J. WeismüllerHanns-ulrich MarschallMarcel JanseDaniel GotthardtChristoph SchrammKristian HolmCisca WijmengaEuijung RyuJohn F. BainesJohannes R. HovJohannes R. HovKirsten Muri BobergEspen MelumEspen MelumHeiko RunzMartina SterneckAndre FrankeAlexey ShiryaevAlexey ShiryaevH-erich WichmannKonstantinos N. LazaridisOle Petter F. ClausenOle Petter F. ClausenChristian RustEva EllinghausStefan SchreiberJon K. LaerdahlJon K. LaerdahlChristian GiegerMichael P. MannsBrian D. JuranPhilipp RauschRinse K. WeersmaSeverine VermeireIsabelle CleynenUlrich BeuersTrine FolseraasTrine FolseraasEinar BjornssonPhilip RosenstielAnnika Bergquistsubject
MaleLinkage disequilibriumendocrine system diseasesGenome-wide association studyPrimary biliary cirrhosisGenotypeBLOOD-GROUPBileChildPOPULATIONAged 80 and overGeneticseducation.field_of_studyPrimary sclerosing cholangitisdigestive oral and skin physiologyMiddle AgedFucosyltransferasesChild PreschoolDISEASESFemaleNeprilysinReceptors Tumor Necrosis Factor Member 14B-LYMPHOCYTEAdultRiskGenome-wide association studyAdolescentGenotypeSUSCEPTIBILITY LOCIFUT2Cholangitis SclerosingPopulationT-LYMPHOCYTE ATTENUATORSingle-nucleotide polymorphismBiologyPolymorphism Single Nucleotidedigestive systemArticlePrimary sclerosing cholangitisGenetic predispositionmedicineImmunogeneticsHumansGenetic Predisposition to DiseaseeducationMETAANALYSISAgedNON-SECRETOR STATUSHepatologymedicine.diseaseGENEdigestive system diseasesSingle nucleotide polymorphismGenetic LociImmunologydescription
Background & Aims: A limited number of genetic risk factors have been reported in primary sclerosing cholangitis (PSC). To discover further genetic susceptibility factors for PSC, we followed up on,a second tier of single nucleotide polymorphisms (SNPs) from a genome-wide association study (GWAS). Methods: We analyzed 45 SNPs in 1221 PSC cases and 3508 controls. The association results from the replication analysis and the original GWAS (715 PSC cases and 2962 controls) were combined in a meta-analysis comprising 1936 PSC cases and 6470 controls. We performed an analysis of bile microbial community composition in 39 PSC patients by 16S rRNA sequencing. Results: Seventeen SNPs representing 12 distinct genetic loci achieved nominal significance (p(replication) <0.05) in the replication. The most robust novel association was detected at chromosome 1p36 (rs3748816; p(combined) = 2.1 x 10(-8)) where the MMEL1 and TNFRSF14 genes represent potential disease genes. Eight additional novel loci showed suggestive evidence of association (p(repl) <0.05). FUT2 at chromosome 19q13 (rs602662; p(comb) = 1.9 x 10(-6), rs281377; p(comb) = 2.1 x 10(-6) and rs601338; p(comb) = 2.7 x 10(-6)) is notable clue to its implication in altered susceptibility to infectious agents. We found that FUT2 secretor status and genotype defined by rs601338 significantly influence biliary microbial community composition in PSC patients. Conclusions: We identify multiple new PSC risk loci by extended analysis of a PSC GWAS. FUT2 genotype needs to be taken into account when assessing the influence of microbiota on biliary pathology in PSC. (C) 2012 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-08-01 |