6533b82ffe1ef96bd1294ef2

RESEARCH PRODUCT

Genotyping of 9 STR systems in combination with 11 diallelic polymorphisms on the Y-chromosome by fragment analysis and minisequencing

subject

description

1. IntroductionThe study of Y chromosomal haplotypes and their relationship to human evolution andvariation is increasing rapidly in the fields of anthropology and forensic genetics.Although autosomal STRs are commonly used and very informative for paternity testingand forensic identification, the use of the nonrecombining portion of the Y chromosome isimportant and provides additional data in cases when the offspring is a male or for mixedmale/female crime stains. For this purpose, in the past 2 years, more and more attentionhas been paid to the examination of diallelic polymorphisms (SNPs) on the Y chromosome[1].Here, we describe an approach for the typing of 10 Y-chromosomal diallelic poly-morphisms (SNPs) using single base extension technology (minisequencing).The frequencies and haplotype relationships of diallelic polymorphisms (Table 1) in asample of 95 individuals of Caucasian origin were examined. In addition to the SNPs, wealso tested the Y-chromosomal diallelic Alu repeat insertion DYS287 (YAP) [2] as well as