6533b82ffe1ef96bd1295a34

RESEARCH PRODUCT

Discovery and Subtyping of Neo-Epitope Specific T-Cell Responses for Cancer Immunotherapy: Addressing the Mutanome

ÖZlem TüreciUgur SahinMathias VormehrMustafa DikenChristian GrunwitzSebastian Kreiter

subject

0301 basic medicineMutationmedicine.diagnostic_testELISPOTmedicine.medical_treatmentT cellCancerBiologymedicine.disease_causemedicine.diseaseSubtypingFlow cytometry03 medical and health sciences030104 developmental biology0302 clinical medicinemedicine.anatomical_structureCancer immunotherapyAntigen030220 oncology & carcinogenesisImmunologymedicine

description

Cancer accumulates 10s to 1000s of genomic mutations of which a fraction is immunogenic and may serve as an Achilles' heel of tumor cells. Mutation-specific T cells can recognize these antigens and destroy malignant cells. Strategies to immunotherapeutically address individual tumor mutations employing peptide or mRNA based vaccines are now actively investigated in mice and humans. An important step of determining the therapeutic potential of a mutanome vaccine is the detection of mutation reactive T-cell responses. In this chapter we provide protocols to identify and subtype mutation specific T cells in mice based on IFN-γ ELISpot and flow cytometry.

yearjournalcountryeditionlanguage
2016-12-17
https://doi.org/10.1007/978-1-4939-6481-9_14