6533b82ffe1ef96bd1295b3c

RESEARCH PRODUCT

Biologically Active Triterpene Saponins from Callus Tissue of Polygala amarella

Marie Aleth Lacaille-duboisYukihiro ShoyamaHildebert WagnerBernard HanquetStéphanie Desbène

subject

Chromatography GasMagnetic Resonance SpectroscopySpectrophotometry InfraredStereochemistryMolecular Sequence DataSaponinPharmaceutical ScienceIn Vitro TechniquesSpectrometry Mass Fast Atom BombardmentAnalytical ChemistryTissue cultureAdjuvants ImmunologicPhagocytosisTriterpeneDrug DiscoveryHumansOleanolic AcidPharmacologychemistry.chemical_classificationPlants MedicinalbiologyChemistryHydrolysisOrganic ChemistryGlycosideSaponinsmusculoskeletal systembiology.organism_classificationTerpenoidEuropecarbohydrates (lipids)Polygala amarellaCarbohydrate SequenceComplementary and alternative medicineBiochemistryCallusSeedsMolecular MedicineSpectrophotometry UltravioletPolygalaceaeGranulocytes

description

A new bioactive saponin (1), together with a known saponin (polygalasaponin XXVIII) has been isolated from the callus tissue culture of Polygala amarella. Based on spectroscopic data, especially direct and long-range heteronuclear 2D NMR analysis and on chemical transformations, the structure of 1 was elucidated as 3-O-beta-D-glucopyranosyl presenegenin-28-O-beta-D-galactopyranosyl-(1 --> 3)-beta-D-xylopyranosyl-(1 --> 4)-alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-glucopyranosyl-(1 --> 3)]-beta-D-fucopyranoside. Both saponins showed significant immunological properties based on the enhancement of granulocyte phagocytosis in vitro.

yearjournalcountryeditionlanguage
1999-07-08Journal of Natural Products
https://doi.org/10.1021/np980577i