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RESEARCH PRODUCT
First morphogenetic analysis of parasite eggs from Schistosomiasis haematobium infected sub-Saharan migrants in Spain and proposal for a new standardised study methodology
M. Isabel Cabeza-barreraAlejandra De Elias-escribanoM. Carmen Oliver-chivaJérôme BoissierPatricio ArtigasSantiago Mas-comaJoaquín Salas-coronasM. Adela ValeroM. Dolores BarguesMarta Reguera-gomezsubject
Sub saharanRange (biology)Veterinary (miscellaneous)Eggs030231 tropical medicinePopulationZoologySchistosomiasisbovisS. haematobium S. bovis030308 mycology & parasitologyhaematobium SStandardised methodology03 medical and health sciencesSchistosomiasis haematobia0302 clinical medicineIntergenic regionPhenotypic analysis[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesparasitic diseasesmedicineParasite hostingAnimalsHumans[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyS. mansonieducationAfrica South of the SaharaSchistosomaOvumTransients and Migrants0303 health scienceseducation.field_of_studybiologySTropical diseaseSub-Saharan migrantsmansonibiology.organism_classificationmedicine.disease3. Good healthInfectious DiseasesSpainInsect ScienceSchistosoma haematobiumParasitologydescription
International audience; Schistosomiasis is a Neglected Tropical Disease caused by trematode species of the genus Schistosoma. Both, autochthonous and imported cases of urogenital schistosomiasis have been described in Europe. The present study focuses on eggs, considered pure S. haematobium by genetic characterisation (intergenic ITS region of the rDNA and cox1 mtDNA). A phenotypic characterisation of S. haematobium eggs was made by morphometric comparison with experimental populations of S. bovis and S. mansoni, to help in the diagnosis of S. haematobium populations infecting sub-Saharan migrants in Spain. Analyses were made by Computer Image Analysis System (CIAS) applied on the basis of new standardised measurements and geometric morphometric tools. The principal component analysis (PCA), including seventeen non-redundant measurements, showed three phenotypic patterns in eggs of S. haematobium, S. bovis and S. mansoni. PCA showed that the S. bovis population presented a large egg size range with a pronouncedly larger maximum size. Similarly, S. bovis shows bigger spine values than S. haematobium. Mahalanobis distances between each pair of groups were calculated for each discriminant analysis performed. In general, S. mansoni and S. bovis present larger distances between them than with S. haematobium, i.e. they present the greatest differences. Regarding the spine, S. haematobium and S. mansoni are the most distant species. Results show the usefulness of this methodology for the phenotypic differentiation between eggs from these Schistosoma species, capable of discerning morphologically close eggs, as is the case of the haematobium group. Schistosoma egg phenotyping approaches may be applied to assess not only hybrid forms but also potential influences of a variety of other factors
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-11-01 |