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RESEARCH PRODUCT
Pathogens and host immunity in the ancient human oral cavity.
Christian Von MeringToshihisa KawaiJessica HendyRoger SeilerJoão Domingos RodriguesChristina WarinnerKai Yik TeohJosé Alfredo Samaniego CastruitaSophy CharltonAnita RadiniRounak VyasCamilla SpellerEske WillerslevM. Thomas P. GilbertChristian D. KelstrupMatthew J. CollinsChristian TrachselSimon Barkow-oesterreicherJonas GrossmannDomingo C. Salazar-garcíaSarah FiddymentHans U. LuderNatallia ShvedElisabeth EpplerRaul Y. TitoPaolo NanniEnrico CappelliniFrank J RühliLars Hestbjerg HansenCecil M. LewisY. HancockJesper V. Olsensubject
ProteomeMolecular Sequence Data610 Medicine & health10071 Functional Genomics Center ZurichDental plaqueArticlePrehistòriaBacterial geneticsPeriodontal pathogenMicrobiology1311 GeneticsTandem Mass SpectrometryGermanyRNA Ribosomal 16SGeneticsmedicineTannerella forsythiaHumansDental CalculusMicrobiomePathogenPhylogenyMouthbiologyBase SequenceEcologyBacteroidetesMicrobiotaSequence Analysis DNAbiology.organism_classificationmedicine.diseaseRed complexHistory Medieval10182 Institute of Oral Biologystomatognathic diseasesArchaeology10076 Center for Integrative Human Physiology11294 Institute of Evolutionary Medicine570 Life sciences; biologyOral MicrobiomeFood AnalysisGenome Bacterialdescription
Calcified dental plaque (dental calculus) preserves for millennia and entraps biomolecules from all domains of life and viruses. We report the first high-resolution taxonomic and protein functional characterization of the ancient oral microbiome and demonstrate that the oral cavity has long served as a reservoir for bacteria implicated in both local and systemic disease. We characterize: (i) the ancient oral microbiome in a diseased state, (ii) 40 opportunistic pathogens, (iii) the first evidence of ancient human-associated putative antibiotic resistance genes, (iv) a genome reconstruction of the periodontal pathogen Tannerella forsythia, (v) 239 bacterial and 43 human proteins, allowing confirmation of a long-term association between host immune factors, “red-complex” pathogens, and periodontal disease, and (vi) DNA sequences matching dietary sources. Directly datable and nearly ubiquitous, dental calculus permits the simultaneous investigation of pathogen activity, host immunity, and diet, thereby extending the direct investigation of common diseases into the human evolutionary past.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-02-01 | Nature genetics |