6533b831fe1ef96bd12984a7
RESEARCH PRODUCT
Cannabinoid control of brain bioenergetics: Exploring the subcellular localization of the CB1 receptor
Giovanni MarsicanoGiovanni MarsicanoPedro GrandesRodrigue RossignolPier Vincenzo PiazzaPier Vincenzo PiazzaLeire RegueroNagore PuenteGiovanni BenardGiovanni BenardBeat LutzFrancis ChaouloffFrancis ChaouloffEtienne Hebert-chatelainsubject
CB1 receptorWIN WIN55212-2Cannabinoid receptorBrain bioenergeticsLactate dehydrogenase Amedicine.medical_treatmentSDHADMSO dimethyl sulfoxideMitochondrionBiologySlp2 stomatin-like protein 2SDHA succinate dehydrogenase aTechnical ReportmedicineantibodieseducationReceptorKO knock-outMolecular Biologyeducation.field_of_studyelectron microscopyLDHa lactate dehydrogenase aDAB–Ni Ni-intensified 33ʹ-diaminobenzidine–4HClCell BiologySubcellular localizationWT wild-typemitochondriaBiochemistryCB1 cannabinoid type 1 receptorBSA bovine serum albuminCannabinoidorganelle purificationNeuroscienceImmunostainingdescription
Brain mitochondrial activity is centrally involved in the central control of energy balance. When studying mitochondrial functions in the brain, however, discrepant results might be obtained, depending on the experimental approaches. For instance, immunostaining experiments and biochemical isolation of organelles expose investigators to risks of false positive and/or false negative results. As an example, the functional presence of cannabinoid type 1 (CB1) receptors on brain mitochondrial membranes (mtCB1) was recently reported and rapidly challenged, claiming that the original observation was likely due to artifact results. Here, we addressed this issue by directly comparing the procedures used in the two studies. Our results show that the use of appropriate controls and quantifications allows detecting mtCB1 receptor with CB1 receptor antibodies, and that, if mitochondrial fractions are enriched and purified, CB1 receptor agonists reliably decrease respiration in brain mitochondria. These data further underline the importance of adapted experimental procedures to study brain mitochondrial functions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-07-01 | Molecular Metabolism |