6533b831fe1ef96bd12986b5

RESEARCH PRODUCT

Optimised method for the analysis of phenolic compounds from caper (Capparis spinosa L.) berries and monitoring of their changes during fermentation

Marcella BarberaFilippo SaianoRaimondo GaglioGiancarlo MoschettiLuca SettanniAlessandra MartoranaMaria AponteNicola Francesca

subject

0301 basic medicineCapparisPolyphenolRutin030106 microbiologySettore AGR/13 - Chimica AgrariaLactobacillus pentosusMass SpectrometryAnalytical Chemistry03 medical and health scienceschemistry.chemical_compoundRutin0404 agricultural biotechnologyfoodYeastsCaper berries Fermentation HPLC–ESI–MS Lactobacillus pentosus Polyphenols Starter culturesBotanyHPLC-ESI-MSFood scienceChromatography High Pressure LiquidbiologyCapparis spinosaLactobacillus pentosufood and beveragesPolyphenols04 agricultural and veterinary sciencesGeneral Medicinebiology.organism_classification040401 food sciencefood.foodCaper berrieAureobasidium pullulansCapparisLactobacillusStarter culturechemistryPolyphenolFruitFermentationCaper berries; Fermentation; HPLC-ESI-MS; Lactobacillus pentosus; Polyphenols; Starter cultures; Food Science; Analytical ChemistryFermentationQuercetinFood ScienceSettore AGR/16 - Microbiologia Agraria

description

In this work, an ad hoc method to identify and quantify polyphenols from caper berries was developed on high-performance liquid chromatography/electrospray ionisation source/mass spectrometry (HPLC-ESI-MS). The method was applied during fermentation carried out with Lactobacillus pentosus OM13 (Trial S) and without starter (Trial C). A total of five polyphenols were identified. All samples contained high concentrations of rutin. Epicatechin was found in untreated fruits, on the contrary quercetin was detected during fermentation. Trial S was characterised by a more rapid acidification and lower levels of spoilage microorganisms than Trial C. L. pentosus dominated among the microbial community of both trials and the highest biodiversity, in terms of strains, was displayed by Trial C. Aureobasidium pullulans was the only yeast species found. The analytical method proposed allowed a high polyphenolic compound recovery from untreated and processed caper berries in short time. The starter culture reduced the bitter taste of the final product.

yearjournalcountryeditionlanguage
2016-01-01
10.1016/j.foodchem.2015.10.045http://hdl.handle.net/10447/154411