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RESEARCH PRODUCT

Simultaneous Determination of Thiamin and Riboflavin in Mushrooms by Liquid Chromatography

María-josé EsteveJosé-manuel García-cantabellaA. FrígolaRosaura Farré

subject

MushroomMeasurement methodChromatographyChemistryRiboflavinExtraction (chemistry)RiboflavinGeneral ChemistryChromatographic separationchemistry.chemical_compoundSpectrometry FluorescenceSpherisorb ODSPhase gradientMethanolAgaricalesGeneral Agricultural and Biological SciencesChromatography Liquid

description

A simple, fast, inexpensive, and reliable method useful for the simultaneous, routine determination of thiamin and riboflavin in mushrooms is examined. It uses the extraction procedure, with slight modifications, proposed by the AOAC for the extraction of thiamin and riboflavin, followed by a liquid chromatographic separation on a reversed-phase Spherisorb ODS column with methanol/water as mobile phase gradient. Fluorometric detection is used at the following excitation and emission wavelengths, respectively, 360 and 425 nm in the case of thiamin and 422 and 515 nm for riboflavin. The analytical parameters of linearity, the precision of the method (RSD = 2.45 and 2.51% for thiamin and riboflavin, respectively), and the results of the comparison with the corresponding AOAC fluorometric methods show that the studied method is useful for the measurement of thiamin and riboflavin in fresh mushrooms. Keywords: Mushrooms; thiamin; riboflavin; liquid chromatographic separation

https://doi.org/10.1021/jf001040p