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RESEARCH PRODUCT
Nuclear aggregation of olfactory receptor genes governs their monogenic expression.
Stavros LomvardasCarolyn A. LarabellCarolyn A. LarabellGilad BarneaFiona G. ClowneyMark LegrosMark LegrosEirene C. Markenskoff-papadimitriouColleen P. MosleyE. Josephine ClowneyMarkko Myllyssubject
Transcription GeneticCytoplasmic and NuclearChromosomal Proteins Non-HistoneDown-RegulationReceptors Cytoplasmic and NuclearLamin B receptorBiologyReceptors OdorantMedical and Health SciencesGeneral Biochemistry Genetics and Molecular BiologyFluorescenceOlfactory Receptor NeuronsArticle03 medical and health sciencesMice0302 clinical medicineDownregulation and upregulationGeneticTranscription (biology)HeterochromatinGene expressionReceptorsmedicineGeneticsAnimalsGeneIn Situ HybridizationIn Situ Hybridization Fluorescence030304 developmental biologyRegulation of gene expressionCell Nucleus0303 health sciencesOlfactory receptorBiochemistry Genetics and Molecular Biology(all)Neurosciencesta1182Non-HistoneBiological SciencesCell biologyChromosomal Proteinsmedicine.anatomical_structureOdorantGene Expression RegulationEctopic expressionTranscription030217 neurology & neurosurgeryDevelopmental Biologydescription
SummaryGene positioning and regulation of nuclear architecture are thought to influence gene expression. Here, we show that, in mouse olfactory neurons, silent olfactory receptor (OR) genes from different chromosomes converge in a small number of heterochromatic foci. These foci are OR exclusive and form in a cell-type-specific and differentiation-dependent manner. The aggregation of OR genes is developmentally synchronous with the downregulation of lamin b receptor (LBR) and can be reversed by ectopic expression of LBR in mature olfactory neurons. LBR-induced reorganization of nuclear architecture and disruption of OR aggregates perturbs the singularity of OR transcription and disrupts the targeting specificity of the olfactory neurons. Our observations propose spatial sequestering of heterochromatinized OR family members as a basis of monogenic and monoallelic gene expression.PaperClip
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-11-01 | Cell |