6533b832fe1ef96bd129a547
RESEARCH PRODUCT
Flow analysis-spectrophotometric determination of ?-dopa in pharmaceutical formulations by reaction with p-Aminophenol
De La Guardia MB. A. HasanKarim D. Khalafsubject
Detection limitChromatographymedicine.diagnostic_testChemistryP-AminophenolRelative standard deviationDosage formAnalytical ChemistryQuinonechemistry.chemical_compoundSpectrophotometrymedicineDerivatizationQuantitative analysis (chemistry)description
Abstract A new method has been developed for the spectrophotometric determination of l -dopa in pharmaceutical formulations. The method is based on the reaction between the open-chain quinone of l -dopa, obtained in NaOH, and the benzoqinoneimine form of p-aminophenol, in the presence of KIO4. The reaction product is determined at 574 nm by using both alternately procedures, one based on the stopped-flow and another on a flow injection approach. Under the best experimental conditions L-dopa can be determined with a limit of detection of 52 ng/ml and a relative standard deviation of 0.2% for three replicate measurements of a solution containing 4 μg/ml.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-08-01 | Talanta |