6533b832fe1ef96bd129a5de

RESEARCH PRODUCT

First identification of polymorphic microsatellite markers in the Burgundy truffle, Tuber aestivum (Tuberaceae)

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SPEIPMCT2Pour cette revue, la version de l'éditeur est autorisée mais il y a un embargo d'un an (mentionné dans les Conditions générales : "On Institutional Repositories after 12 months").On peut donc mettre la PJ en "publique" mais il faut indiquer une date d'embargo (un an à partir de la date de publication). L'embargo sera levé automatiquement. Laissée lors du dépôt en workflow par prudence.; Premise of the study: Tuber aestivum, the most common truffle in Europe, plays an important role in the commercial truffle market. For the first time, microsatellite primers were developed to investigate polymorphism within this species. • Methods and Results: Using direct shotgun pyrosequencing, 15 polymorphic microsatellites were identified out of the 7784 perfect microsatellites present in the 534 620 reads obtained. Tested on 75 samples, these microsatellites were highly polymorphic. The number of alleles varied from four to 15, and the expected heterozygosity ranged from 0.266 to 0.620. A multilocus analysis allowed the identifi cation of 63 genotypes over the 75 samples analyzed. • Conclusions: Direct shotgun pyrosequencing is a fast and relatively low-cost technique allowing identifi cation of microsatellites in nonmodel species. The microsatellites developed in this study will be useful in population genetic studies to infer the evolutionary history of this species.