6533b832fe1ef96bd129ad47
RESEARCH PRODUCT
Children monosensitized to pine nuts have similar patterns of sensitization
Patrizia RestaniNeri PucciFrancesca MoriElio NovembreElena PeñasCinzia BallabioGiuliana FerranteFrancesca UbertiSimona Barnisubject
MaleNutVeterinary medicinepine nuts allergyanimal structuresAdolescentUrticariaImmunologyProtein profileImmunoglobulin Ecomplex mixtureschildrenBotanyHumansNutsImmunology and AllergyMedicineAngioedemaSensitizationPlant ProteinsSkin Testsbiologybusiness.industryfungidigestive oral and skin physiologyfood and beveragesImmunoglobulin Emedicine.diseasemedicine.anatomical_structurePediatrics Perinatology and Child Healthbiology.proteinFemaleNut HypersensitivityNut allergiesbusinessimmunoblottingAnaphylaxisdescription
Background Several cases of pine nut allergies and anaphylaxis have been reported in the literature, but only few pine nut allergens have been characterized. The aim of this research is to identify through immunoelectrophoretic techniques the major pine nut allergens in a group of children monosensitized to pine nuts. Methods We studied five children with pine nut allergies and no other sensitization to food except to pine nuts, confirmed by in vivo (prick test, prick-to-prick) and in vitro tests (specific IgE determinations [CAP-FEIA]). The protein profile of pine nuts was analyzed by Sodium Dodecyl sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Immunoblotting was performed after incubation of membranes with the sera from the children included in the present study. Results Immunoblotting (SDS-PAGE) demonstrated five similar bands between 6 and 47 kDa in all the subjects studied. Conclusion These bands should be considered the potential allergens for pine nut allergic children.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 |