6533b832fe1ef96bd129af54

RESEARCH PRODUCT

Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain

Arturo Alvarez-buyllaJosé Manuel García-verdugoErik M. ShapiroAlan P. KoretskyOscar Gonzalez-perez

subject

Doublecortin ProteinRostral migratory streamCognitive NeuroscienceSubventricular zoneArticleCerebral VentriclesRats Sprague-DawleyCell MovementmedicineAnimalsProgenitor cellProgenitorNeuronsbiologyChemistryStem CellsBrainImmunohistochemistryMagnetic Resonance ImagingOlfactory BulbNeural stem cellRatsDoublecortinOlfactory bulbCell biologyMicroscopy Electronmedicine.anatomical_structurenervous systemNeurologybiology.proteinStem cellNeuroscienceFerrocyanides

description

Neural progenitor cells (NPCs) reside within the subventricular zone (SVZ) in rodents. These NPCs give rise to neural precursors in adults that migrate to the olfactory bulb (OB) along a well-defined pathway, the rostral migratory stream (RMS). Here we demonstrate that these NPCs can be labeled, in vivo, in adult rats with fluorescent, micron-sized iron oxide particles (MPIOs), and that magnetic resonance imaging (MRI) can detect migrating neural precursors carrying MPIOs along the RMS to the OB. Immunohistochemistry and electron microscopy indicated that particles were inside GFAP(+) neural progenitor cells in the SVZ, migrating PSA-NCAM(+) and Doublecortin(+) neural precursors within the RMS and OB, and Neu-N(+) mature neurons in the OB. This work demonstrates that in vivo cell labeling of progenitor cells for MRI is possible and enables the serial, non-invasive visualization of endogenous progenitor/precursor cell migration.

yearjournalcountryeditionlanguage
2006-09-01NeuroImage
https://doi.org/10.1016/j.neuroimage.2006.04.219