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RESEARCH PRODUCT
Chemoselektive Fluoreszenzstudien am Subtilisin und “Thiosubtilisin”
Leopold HornerHans-wolfram Flemmingsubject
biologyStereochemistryfungiOrganic ChemistryHydroxy groupSubtilisinActive siteVinyl sulfoneFluorescenceSerineenzymes and coenzymes (carbohydrates)chemistry.chemical_compoundchemistrybiology.proteinOrganic chemistryPhysical and Theoretical ChemistryFluoridedescription
Chemoselective Fluorescence Studies with Subtilisin and “Thiosubtilisin” The serine hydroxy group in the active site of subtilisin is selectively “phosphylated”3). This is demonstrated using the fluorescent phosphinic fluoride 1. “Thiosubtilisin” does not react with 1 but with the SH-selective and fluorescent vinyl sulfone 2.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1985-02-12 | Liebigs Annalen der Chemie |