6533b834fe1ef96bd129ceb4
RESEARCH PRODUCT
Oleanane-type glycosides from the roots of Weigela florida “rumba” and evaluation of their antibody recognition
Chiaki TanakaAnne Sophie Champy-tixierAnne Sophie Champy-tixierMarie Aleth Lacaille-duboisFeliciana Real-fernándezAnna Maria PapiniTomofumi MiyamotoAnne Claire Mitaine-offersubject
0301 basic medicineMultiple SclerosisStereochemistryEnzyme-Linked Immunosorbent AssayCaprifoliaceaePlant Roots01 natural sciences03 medical and health scienceschemistry.chemical_compoundTriterpeneDrug DiscoveryHumansGlycosidesOleanolic AcidCaprifoliaceaeOleanolic acidOleananePharmacologychemistry.chemical_classificationMolecular Structurebiology010405 organic chemistryGlycosideGeneral Medicinebiology.organism_classification0104 chemical sciences030104 developmental biologyImmunoglobulin MchemistryImmunoglobulin Mbiology.proteinAntibodyTwo-dimensional nuclear magnetic resonance spectroscopydescription
Three triterpene glycosides were isolated from the roots of Weigela florida "rumba" (Bunge) A. DC.: two previously undescribed 3-O-β-d-xylopyranosyl-(1→2)-[β-d-xylopyranosyl-(1→4)]-β-d-xylopyranosyl-(1→4)-β-d-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-l-arabinopyranosyloleanolic acid (1) and 3-O-β-d-xylopyranosyl-(1→2)-[β-d-glucopyranosyl-(1→4)]-β-d-xylopyranosyl-(1→4)-β-d-xylopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyloleanolic acid (2), and one isolated for the first time from a natural source 3-O-β-d-xylopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→2)-α-l-arabinopyranosyloleanolic acid (3). Their structures were elucidated mainly by 2D NMR spectroscopic analysis (COSY, TOCSY, NOESY, HSQC, HMBC) and mass spectrometry. Compounds 2 and 3 were further evaluated as antigens in enzyme-linked immunosorbent assay (ELISA) to recognize IgM antibodies in multiple sclerosis (MS) patients' sera.
year | journal | country | edition | language |
---|---|---|---|---|
2018-07-01 | Fitoterapia |