6533b834fe1ef96bd129d670
RESEARCH PRODUCT
Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products
Doina MiereJordi MañesCristina JuanOana StanciuFelicia Loghinsubject
Electrospraymethod validation ; productos elaborados con trigo ; LC-MS/MS ; Emerging mycotoxins ; Microtoxinas emergentes ; validación ; cereal-based productsGeneral Chemical Engineeringlcsh:TX341-641Tandem mass spectrometryQuechersIndustrial and Manufacturing EngineeringMatrix (chemical analysis)chemistry.chemical_compound0404 agricultural biotechnologyLc ms msLC-MS/MSMycotoxinChromatographylcsh:TP368-456Extraction (chemistry)method validation04 agricultural and veterinary sciencesGeneral Chemistry040401 food scienceBeauvericinlcsh:Food processing and manufacturechemistryEmerging mycotoxinslcsh:Nutrition. Foods and food supplyFood Sciencecereal-based productsdescription
Due to the matrix complexity for wheat-based products, a comparative study of different rapid extraction procedures was performed for the extraction of enniatins (ENA, ENA1, ENB, ENB1) and beauvericin in flour, pasta, breakfast cereals, and biscuits. Three different approaches were studied during the extraction and purification steps (shaker, Ultra-Turrax, and QuEChERS) for each matrix. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray source working in a positive mode was used. For the analysis of the five mycotoxins, the three methods were tested in terms of recovery, matrix effect, and sensibility, concluding that Ultra-Turrax extraction was the most competent method. The applicability of the validated method was demonstrated by analyzing 16 commercial samples from Romania. Debido a la complejidad de los productos elaborados con trigo, se ha realizado un estudio comparativo de diferentes procesos de extracción rápida de eniatinas (ENA, ENA1, ENB, ENB1) y beauvericina en harina, pasta, cereales para el desayuno y galletas. Se estudiaron tres procedimientos (agitación, Ultra-Turrax, QuEChERS) en cada matriz con distintos pasos de extracción y purificación. Se utilizó la cromatografía líquida acoplada a espectrometría de masas en tándem (LC-MS/MS) con fuente de electroespray en modo positivo. Los tres métodos fueron estudiados para el análisis de las cinco micotoxinas, en términos de recuperación, efecto matriz y sensibilidad, concluyendo que el método de extracción con Ultra-Turrax fue el que mejores resultados proporcionó. La aplicabilidad del método validado se demostró con el análisis de 16 muestras comercializadas en Rumania.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-02-15 |