6533b834fe1ef96bd129e168

RESEARCH PRODUCT

Assessment of induced corneal hypoxia in diabetic patients

Philippe D’athisPhilippe MoirouxJean-marcel BrunCatherine Creuzot-garcherAlain M. BronMehdi Ziadi

subject

AdultMalemedicine.medical_specialtyPathologyCell CountCorneachemistry.chemical_compoundStress PhysiologicalOphthalmologyLactate dehydrogenaseDiabetes mellitusHumansMedicineIn patient[SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory OrgansHypoxiaAged[SDV.MHEP] Life Sciences [q-bio]/Human health and pathologyL-Lactate Dehydrogenasebusiness.industryCorneal EdemaEndothelium CornealMiddle AgedHypoxia (medical)Contact Lenses Hydrophilicmedicine.diseaseeye diseasesOphthalmologyDiabetes Mellitus Type 1Diabetes Mellitus Type 2chemistry[SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory OrgansTearsTearsFemaleCorneal hypoxiasense organsmedicine.symptomSwellingbusinessAnaerobic exercise[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

description

Comparative Study Address correspondence and reprint requests to Dr. C. Creuzot-Garcher, Service d'Ophtalmologie, CHU, Hopital Général, 3 Rue du Faubourg Raines, BP 1519, 21033 Dijon, France. E-mail: Catherine.creuzot-garcher@chu-dijon.fr; To assess the control of corneal hydration in patients with diabetes during a contact lens-induced hypoxia. Corneal stress was induced in 15 patients with diabetes and 23 healthy patients by having them wear contact lenses for 2 hours. Pachymetries were measured and corneal parameters (percentage recovery per hour [PRPH], time for deswelling [T99%] and induced swelling [IS]) were calculated. In the mean time, tears were collected to assess the activity of lactate dehydrogenase (LDH), and a specular microscopy (SM) was performed. In patients with diabetes, PRPH, T99%, and LDH activity were statistically significantly modified (p < 0.05), whereas IS and SM were not. This decreased ability to recover the initial corneal thickness after a transient edema caused by hypoxia confirms the enzymatic dysfunction of the endothelial pumps that are partly caused by a shift toward anaerobic metabolism.

yearjournalcountryeditionlanguage
2002-01-01
https://hal.inrae.fr/hal-02676615