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RESEARCH PRODUCT
Meat allergy associated with galactosyl‐α‐(1,3)‐galactose (α‐Gal)—Closing diagnostic gaps by anti‐α‐Gal IgE immune profiling
A KrommingaW AnemüllerT GoldmannFranziska RuëffUta JappeI WaldmannA HomannNicola WagnerRegina TreudlerP SeidelA. WalkerHans F. MerkA LudwigThomas Ae Platts-millsBernhard PrzybillaWolf-meinhard BeckerJoachim SalogaS MingeBurkhard KreftTilo BiedermannTilo BiedermannRita VargaMargitta Wormsubject
AdultMale0301 basic medicineAllergyMeatmedicine.medical_treatmentImmunologyDot blotCross ReactionsImmunoglobulin EArticleYoung Adult03 medical and health sciences0302 clinical medicinemedicineHumansImmunology and AllergyMultiplexAnaphylaxisSensitizationAgedbiologybusiness.industryImmunochemistryGalactoseAllergensImmunoglobulin EMiddle Agedmedicine.diseaseRed Meat030104 developmental biologymedicine.anatomical_structure030228 respiratory systemCase-Control StudiesImmunologyMonoclonalbiology.proteinFemaleThyroglobulinbusinessFood HypersensitivityAnaphylaxisdescription
Background Glycoproteins and glycolipids of some mammalian species contain the disaccharide galactosyl-α-(1,3)-galactose (α-Gal). It is known that α-Gal is immunogenic in humans and causes glycan-specific IgG and also IgE responses with clinical relevance. α-Gal is part of the IgE-reactive monoclonal therapeutic antibody cetuximab (CTX) and is associated with delayed anaphylaxis to red meat. In this study, different α-Gal-containing analytes are examined in singleplex and multiplex assays to resolve individual sensitization patterns with IgE against α-Gal. Methods Three serum groups, α-Gal-associated meat allergy (MA) patients, idiopathic anaphylaxis (IA) patients with suspected MA, and non-meat-allergic healthy control individuals (HC), were analyzed via singleplex allergy diagnostics and a newly established immunoblot diagnostic system. The new dot blot detection system resolved individual IgE sensitization profiles for α-Gal-containing analytes CTX, bovine thyroglobulin (Bos d TG), and human serum albumin (HSA)-conjugated α-Gal. Results Singleplex allergy diagnostics using the α-Gal analytes CTX and Bos d TG confirms the history of MA patients in 91% and 88% of the cases, respectively. A novel dot blot-based assay system for the detection of IgE against α-Gal reveals individual IgE sensitization profiles for α-Gal-containing analytes. An α-Gal-associated IgE cross-reactivity profile (IgE against CTX, Bos d TG, and HSA-α-Gal) was identified, which is associated with MA. Conclusions Detection of individual sensitization patterns with different α-Gal-containing analytes provides the basis for an individual allergy diagnosis for α-Gal-sensitized patients. Higher amounts of α-Gal in pork and beef innards compared to muscle meat as indicated by a higher staining intensity are a plausible explanation for the difference in allergic symptom severity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-08-15 | Allergy |