6533b835fe1ef96bd129e967

RESEARCH PRODUCT

Prognostic role of immune checkpoint-related genes in resectable lung adenocarcinomas.

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11085 Background: Immune checkpoints blockade has demonstrated promising clinical results in NSCLC patients. In this study we have investigated the prognostic role of immune checkpoint markers (CTLA4, PD1 and PDL1) in resectable lung adenocarcinoma (ADC). Methods: RNA was isolated from fresh-frozen lung specimens (tumor and normal lung) from resectable ADC patients (n=92). RT-PCR was performed to analyze the expression of CTLA4, PD1 and PDL1 by the use of hydrolysis probes. Relative gene expression was assessed by Pfaffl formula and normalized by CDKN1B and ACTB as endogenous genes (selected by geNorm algorithm). Statistical analyses were considered significant at p<0.05. Results: Patient’s median age was 64 [37-82], 77.2% were male, 73.9 % have a performance status of 0 and 58.7% presented stage I at the time of the diagnosis. We found a significant positive correlation between expression levels of PD1 and PDL1 (r2=0.436; p<0.0001) and between PD1 and CTLA4 (r2=0.458; p<0.0001). Survival analyses reveale...