6533b835fe1ef96bd129fef7
RESEARCH PRODUCT
The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors
Collesano MDavide CoronaSwonild Ilenia GenoveseAntonia M. R. IngrassiaMaria Cristina OnoratiWalter ArancioGiosalba BurgioManolis Fantosubject
Chromatin Remodeling FactorInvestigationsBiologyEyemedicine.disease_causeConserved sequenceEvolution MolecularGeneticsmedicineAnimalsDrosophila ProteinsNucleosomeFluorometryGenetic TestingGenes SuppressorTranscription factorConserved SequenceAdenosine TriphosphatasesGeneticsMutationCell CycleDNA replicationbiology.organism_classificationNucleosomesChromatinDrosophila melanogasterPhenotypeMutationBiological AssayDrosophila melanogasterchromatin drosophila ISWIProtein BindingTranscription Factorsdescription
Abstract ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic and biochemical analyses.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 |