6533b835fe1ef96bd12a0132
RESEARCH PRODUCT
Mepanipyrim haptens and antibodies with nanomolar affinity
Antonio Abad-somovillaConsuelo AgullóFrancesc A. Esteve-turrillasAntonio Abad-fuentesJosep V. Mercadersubject
AnalyteAntibody AffinityHeterologousBiochemistryAntibodiesAnalytical ChemistryElectrochemistryAnimalsEnvironmental ChemistryMepanipyrimIC50SpectroscopyA determinantImmunoassayChromatographybiologyChemistryAntibody productionPyrimidinesBiochemistrybiology.proteinFemaleRabbitsAntibodyHaptensHaptenConjugatedescription
Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC50 = 3 nM). © 2013 The Royal Society of Chemistry.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-05-14 | The Analyst |