6533b836fe1ef96bd12a0748

RESEARCH PRODUCT

Der cytochemische Nachweis von Prolin-Dehydrogenasen, Acetaldehyd-Dehydrogenasen und Dihydrolipons�ure-Dehydrogenase in den Zellen vonSaccharomyces cerevisiae

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Using the tetrazolium salt Nitro-BT, the following dehydrogenases can be demonstrated cytochemically in the cells ofSaccharomyces cerevisiae: (1)Proline dehydrogenase activity: it cannot be decided whether the formazan production is a result of L-proline: NAD(P)-2-oxidoreductase (E.C. 1.5.1.1) or of L-proline:NAD(P)-5-oxidoreductase(E.C. 1.5.1.2); (2)Aldehyde dehydrogenase activity: using the coenzymes NAD and NADP and the activators KCl and MgCl2, different reaction pictures are obtained which led to the conclusion that aldehyde: NADP oxidoreductase (E.C. 1.2.1.4) and aldehyde: NAD(P) oxidoreductase (E.C. 1.2.1 5) can be demonstrated seperately; (3)Dihydrolipoic dehydrogenase (E.C. 1.6.4.3): an exactly controlled pH level (pH 6.0) of the complete incubation medium is an essential prerequisite for the specificity of the reaction. The formation of filamentous formazan deposits can be interpreted as the expression of the mitochondrial localization of the enzyme.