6533b836fe1ef96bd12a0822
RESEARCH PRODUCT
Bioreducible Poly-l-Lysine-Poly[HPMA] Block Copolymers Obtained by RAFT-Polymerization as Efficient Polyplex-Transfection Reagents
Kristof TappertzhofenEvelyn MontermannKaloian KoynovMatthias BrosRudolf ZentelDavid HuesmannSimone BeckMatthias Barzsubject
Polymers and PlasticsCationic polymerizationBioengineering02 engineering and technologyTransfection010402 general chemistry021001 nanoscience & nanotechnology01 natural sciences0104 chemical sciencesBiomaterialschemistry.chemical_compoundchemistryPolymerizationPolylysinePolymer chemistryMaterials ChemistryCopolymerMoietyReversible addition−fragmentation chain-transfer polymerization0210 nano-technologyProtecting groupBiotechnologydescription
Polylysine-b-p[HPMA] block copolymers containing a redox-responsive disulfide bond between both blocks are synthesized by RAFT polymerization of pentafluorphenyl-methacrylate with a macro-CTA from Nϵ-benzyloxycarbonyl (Cbz) protected polylysine (synthesized by NCA polymerization). This polylysine-b-p[PFMA] precursor block copolymer is converted to polylysine(Cbz)-b-p[HPMA] by postpolymerization modification with 2-hydroxypropylamine. After removal of the Cbz protecting group, cationic polylysine-b-p[HPMA] copolymers with a biosplittable disulfide moiety became available, which can be used as polymeric transfection vectors. These disulfide linked polylysine-S-S-b-p[HPMA] block copolymers show low cytotoxicity and increased transfection efficiencies (HEK-293T cells) compared to analogous blockcopolymers without disulfide group making them interesting for the transfection of sensitive immune cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-07-29 | Macromolecular Bioscience |