6533b836fe1ef96bd12a1b86
RESEARCH PRODUCT
Synthesis of Maleimide-Functionalyzed HPMA-Copolymers and in vitro Characterization of the aRAGE- and Human Immunoglobulin (huIgG)-Polymer Conjugates
Mario HuboVerena V. MetzRolf PostinaMatthias BarzKristof TappertzhofenHelmut JonuleitRudolf Zentelsubject
Polymers and PlasticsbiologyDispersityBioengineeringChain transferRaftBiomaterialschemistry.chemical_compoundchemistryPolymerizationPolyclonal antibodiesPolymer chemistryMaterials Chemistrybiology.proteinMethacrylamideMaleimideBiotechnologyConjugatedescription
Herein the synthesis of antibody–polymer conjugates, with a quite narrow dispersity based on the polymer HPMA, are reported. These conjugates are synthesized by coupling antibodies to maleimide-functionalized poly(N-(2-hydroxypropyl)-methacrylamide) (poly-HPMA) copolymers derived through reversible addition-fragmentation chain transfer (RAFT) polymerization of pentafluorophenyl methacrylate via the intermediate step of an activated ester polymer. We develop a protocol that allows the attachment of two different model antibodies, monoclonal anti-RAGE (receptor for advanced glycation end-products) antibody, and polyclonal human immunoglobulin (huIgG). Modification of the antibody and conjugation is monitored by SDS-PAGE electrophoresis. Preserved affinity is demonstrated by Western Blott and cell-uptake analysis, for example, to cells of the immune system.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-12-13 | Macromolecular Bioscience |