6533b837fe1ef96bd12a2737
RESEARCH PRODUCT
Determination of arsenic compounds in beverages by high-performance liquid chromatography-inductively coupled plasma mass spectrometry.
E S De LimaM. De La GuardiaNívia M. M. CoelhoA. PastorLuciana Melo Coelhosubject
Detection limitchemistry.chemical_compoundChromatographyCertified reference materialsChemistryArsenous acidchemistry.chemical_elementArsenic CompoundHigh-performance liquid chromatographyArsenic acidInductively coupled plasma mass spectrometryArsenicAnalytical Chemistrydescription
Arsenic compounds including arsenous acid (As(III)), arsenic acid (As(V)), dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were separated by high-performance liquid chromatography (HPLC) and detected by inductively coupled plasma mass spectrometry (ICP-MS). A Hamilton PRX-100 anionic-exchange column and a pH 8.5 K(2)HPO(4)/KH(2)PO(4) 5.0x10(-3)molL(-1) mobile phase were used to achieve arsenic speciation. The separation of arsenic species provided peaks of As(III) at 2.75min, DMA at 3.33min, MMA at 5.17min and As(V) at 12.5min. The detection limits, defined as three times the standard deviation of the lowest standard measurements, were found to be 0.2, 0.2, 0.3 and 0.5ngmL(-1) for As(III), DMA, MMA and As(V), respectively. The relative standard deviation values for a solution containing 5.0mugL(-1) of As(III), DMA, MMA and As(V) were 1.2, 2.1, 2.5 and 3.0%, respectively. This analytical procedure was applied to the speciation of arsenic compounds in drinking (soft drink, beer, juice) samples. The validation of the procedure was achieved through the analysis of arsenic compounds in water and sediment certified reference materials.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-05-15 | Talanta |