6533b839fe1ef96bd12a64b4
RESEARCH PRODUCT
Centrifugation does not alter spatial distribution of `BEP4' mRNA in paracentrotus lividus EGG
Daniele P. RomancinoCaterina CostaCarmela CavalcanteAnna Maria RinaldiMarta Di CarloAiti Vizzinisubject
Localized mRNABiophysicsCentrifugationCell FractionationBiochemistryMicrotubulesParacentrotus lividuschemistry.chemical_compoundStructural BiologyBotanyA/V axisGeneticsCentrifugation Density GradientColchicineAnimalsCentrifugationNorthern blotRNA MessengerMolecular BiologyGeneIn Situ HybridizationOvumMessenger RNAbiologyUnfertilized EggsRNACell PolarityMembrane ProteinsCell Biologybiology.organism_classificationBlotting NorthernMolecular biologychemistrySea Urchinsembryonic structuresCortexColchicinedescription
AbstractParacentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts: a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half. Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-06-30 | FEBS Letters |