6533b839fe1ef96bd12a65ba
RESEARCH PRODUCT
Identification of major histocompatibility complex class II-associated peptides derived from freshly prepared rat Langerhans cells using MALDI-PSD and Edman degradation
Martin BlüggelMichael KulkeAlexandra MarxHelmut E. MeyerKonrad ReskeAlbert SickmannHolger Kremminsubject
Langerhans cellPeptideMass spectrometryBiochemistryMass SpectrometryAnalytical ChemistryMHC class IElectrochemistrymedicineAnimalsEnvironmental ChemistryPeptide sequenceSpectroscopySkinchemistry.chemical_classificationSample handlingChromatographyMajor Histocompatibility Complex Class IIbiologyEdman degradationHistocompatibility Antigens Class IIRatsmedicine.anatomical_structurechemistryBiochemistryRats Inbred LewLangerhans Cellsbiology.proteindescription
The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at −80 °C to prevent peptide losses.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-07-13 |