6533b839fe1ef96bd12a6dba
RESEARCH PRODUCT
Magnetic–fluorescent Langmuir–Blodgett films of fluorophore-labeled ferritin nanoparticles
Miguel Clemente-leónFrancisco Santoyo-gonzalezRafael CuestaAlejandra Soriano-portilloRuperto BermejoJulia Morales-sanfrutosBelén FernándezNatividad GálvezPurificación SánchezJosé M. Domínguez-veraEugenio Coronadosubject
FluorophorebiologyChemistryAnalytical chemistryQuantum yieldNanoparticleGeneral ChemistryCondensed Matter PhysicsPhotochemistryLangmuir–Blodgett filmFluorescenceFerritinchemistry.chemical_compoundbiology.proteinGeneral Materials ScienceLuminescenceSuperparamagnetismdescription
Abstract We have covalently coupled fluorophore 4-(2-hydroxyethoxy)-7-nitro-2,1,3-benzoxadiazole (NBD) to the external ferritin shell through lysine residues. An increase in the luminescence quantum yield of the fluorescent ferritin particles and a blue shift in its emission peak compared to individual fluorophore were observed. The study of the particles by transmission electron microscopy showed that the native iron core ferritin is intact and that no degradation occurs during chemical functionalization of the protein shell. The NBD-labeled ferritin particles are water soluble, which allowed their controlled deposition by the Langmuir–Blodgett (LB) technique. Superparamagnetic and fluorescent properties of the particles are preserved within the LB film.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-04-01 | Solid State Sciences |