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RESEARCH PRODUCT
Halloysite nanotubes for efficient loading, stabilization and controlled release of insulin
Silvia PieracciniSerena RielaGiuseppe LazzaraGiuseppe CavallaroCarmelo Giuseppe CollettiGiuseppe D'azzoMarina MassaroSusanna Guernellisubject
Dichroismmedicine.medical_treatmentHalloysite nanotube02 engineering and technology01 natural sciencesBiochemistryNanocompositesChitosanchemistry.chemical_compoundColloid and Surface ChemistryDrug StabilityProtein stabilityHalloysite nanotube (HNTs)InsulinTransdermalSettore CHIM/02 - Chimica FisicaDrug CarriersNanotubesProteolytic enzymes021001 nanoscience & nanotechnologyControlled releaseSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsEnzyme inhibitionAluminum SilicatesBionanocomposite film0210 nano-technologyHybrid materialBionanocomposite hybridSurface PropertiesDrug Compoundingengineering.materialCircular dichroism data010402 general chemistrySustained release InsulinAdministration CutaneousHalloysiteBiomaterialsKaolinitemedicineParticle SizeHybrid materialChitosanInsulinBiomedical applicationMedical applicationYarn Bio-nanocompositeMembranes Artificial0104 chemical sciencesNanotubeDrug LiberationHalloysite nanotubes Insulin Protein stability Sustained release Bionanocomposite hybridchemistryChemical engineeringDelayed-Action PreparationsengineeringClayNanocarriersSustained releasedescription
Hypothesis: Oral insulin administration is not actually effective due to insulin rapid degradation, inactivation and digestion by proteolytic enzymes which results in low bioavailability. Moreover insulin is poorly permeable and lack of lipophilicity. These limits can be overcome by the loading of protein in some nanostructured carrier such as halloysite nanotubes (HNTs). Experiments: Herein we propose an easy strategy to obtain HNT hybrid materials for the delivery of insulin. We report a detailed description on the thermal behavior and stability of insulin loaded and released from the HNTs hybrid by the combination of several techniques. Findings: Release experiments of insulin from the HNTs revealed the efficacy of the nanocarrier. Circular Dichroism data evidenced that the released insulin exhibits its native-like secondary structure confirming the suitability of HNT/insulin as delivery system for at least three months. The loaded nanotubes were filled into chitosan matrix with the aim to prepare bionanocomposite films that can be used for transdermal delivery. This work puts forward an efficient strategy to prepare halloysite based nanocarriers containing insulin that could be employed in several biomedical applications. The detailed description of the prepared HNT/insulin hybrid represents a fundamental point for designing advanced delivery systems. © 2018 Elsevier Inc.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |