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RESEARCH PRODUCT

249 Validation of assessment of circulate oxidative stress markers by the Free Oxygen Radicals Testing (FORT) assay among patients with an acute myocardial infarction

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BackgroundFree oxygen radicals play an important role in the pathogenesis of many diseases including cardiovascular diseases, diabetes, cancer and aging. Several methods were developed for the direct or indirect measurement of oxygen free radical and its by-products. Using a new Free Oxygen Radicals Testing (FORT) the current study is designed first to validate the device and to investigate the potential relationships between the ROS and clinical or biological factors in human serum from a population of men with an acute myocardial infarction (AMI).MethodsWe first determined the effect of storage, variability and reproducibility of the FORT test in serum. Then we used the test in 66 patients from our bio bank of AMI patients.ResultsFORT values vary between 324 and 1198 FORT units, with a median value of 581 (494–754) FORT units. Among the risk factors, 17% of patients are diabetic, and 20% are obese. In univariate analysis, the FORT values seem to be influenced by age (r = 0.161, p = 0.195), presence of diabetes (p = 0.102), a history of MI (p = 0.181), LVEF <40% (p = 0.005) and treatment with - blockers before admission (p = 0.053), with ST-Elevation MI (p = 0.058), levels of CRP (r = 0.438, p<0.001), the rate of neutrophil (r = 0.203, p = 0.107) and peak CK (r = 0.274, p = 0.028). The analysis of multiple linear regression showed that CRP (p = 0.023), LVEF <40% (p<0.001) and presence of diabetes (p = 0.039) were independent predictors of serum FORT levels. This statistical model can explain 45% of the variance in the FORT levels.ConclusionsThe variability of the FORT on serum is minimal and thus reproducibility can be attained. FORT assay is stable when stored at 20°C for a couple of months or at 4°C for a few days. FORT correlation with CRP, LVEF and status of diabetes provides an interesting insight and a good link between oxidative stress and inflammation in patients with an AMI.