6533b83afe1ef96bd12a71c0

RESEARCH PRODUCT

Porphyrin-bile acid conjugates: from saccharide recognition in the solution to the selective cancer cell fluorescence detection.

Juha KoivukorpiPavla PouckovaJarmila KrálováZdeněk KejíkElina SievänenErkki KolehmainenVladimír Král

subject

GlycosylationPorphyrinsmedicine.drug_classmedicine.medical_treatmentCarbohydratesPhotodynamic therapyApoptosisDNA FragmentationLigandsBiochemistrySensitivity and SpecificityCell LineBile Acids and Saltschemistry.chemical_compoundMiceStructure-Activity RelationshipIn vivoNeoplasmsmedicineFluorescence microscopeBiomarkers TumorAnimalsHumansPhysical and Theoretical ChemistryCell Line TransformedCell ProliferationMice Inbred BALB CBinding SitesBile acidDose-Response Relationship DrugMolecular StructureChemistryOrganic ChemistryCancer3T3 Cellsmedicine.diseasePorphyrinSolutionsCell killingBiochemistryMicroscopy FluorescencePhotochemotherapyCancer cellDrug Screening Assays AntitumorHeLa Cells

description

This paper describes the preparation and use of conjugates of porphyrins and bile acids as ligands to bind to tumor expressed saccharides. Bile acid-porphyrin conjugates were tested for recognition of saccharides that are typically present on malignant tumor cells. Fluorescence microscopy, in vitro PDT cell killing, and PDT of subcutaneous 4T1 mouse tumors is reported. High selectivity for saccharide cancer markers and cancer cells was observed. This in vivo and in vitro study demonstrated high potential use for these compounds in targeted photodynamic therapy.

yearjournalcountryeditionlanguage
2008-04-19Organicbiomolecular chemistry
10.1039/b717528khttps://pubmed.ncbi.nlm.nih.gov/18421385