6533b83afe1ef96bd12a7a54
RESEARCH PRODUCT
Proteomic analysis of Parietaria judaica pollen and allergen profiling by an immunoproteomic approach
Giacomo De LeoGiovanni DuroMichel BecchiSimona TavernaIsabelle Zanella-cléonMarilisa BarrancaRiccardo AlessandroSimona Fontanasubject
ProteomicsAllergyProteomeCellular functionsBioengineeringmedicine.disease_causeProteomicsApplied Microbiology and BiotechnologyAllergenTandem Mass SpectrometryPollenotorhinolaryngologic diseasesmedicineHumansElectrophoresis Gel Two-DimensionalPlant Proteinsbiologyfood and beveragesGeneral MedicineAntigens PlantImmunoglobulin Ebiology.organism_classificationmedicine.diseaseParietariaImmunologyProteomeParietaria judaicaPollenParietaria judaica pollenallergens Parietaria judaica immunoproteome pollenBiotechnologyChromatography Liquiddescription
Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 |