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RESEARCH PRODUCT
Spectrophotometric determination of chlorhexidine with bromocresol green by flow-injection and manual methods
P. Campíns FalcóJ. Martínez Calatayudsubject
ChromatographyBromocresol greenmedicine.diagnostic_testChlorhexidineMolar absorptivityAscorbic acidBiochemistryAnalytical ChemistryBenzocainechemistry.chemical_compoundchemistrySpectrophotometrymedicineEnvironmental ChemistrySpectroscopymedicine.drugdescription
Abstract A spectrophotometric study of the chlorhexidine/bromocresol green/Triton X-100 system is reported; at pH 5.3, both 2:1 and 1:1 bromocresol green/chlorhexidine complexes are formed. In the manual spectrophotometric method, Beer's law is obeyed for chlorhexidine concentrations of 2.9–32.2 μg ml −1 (r.s.d. 0.4–1.3%); the molar absorptivity is 12 500 l mol −1 cm −1 . In the flow-injection method, the calibration graph is linear for the chlorhexidine range 23.0–83.9 μg ml −1 (r.s.d. 0.8%); the injection is ca. 60 h −1 . Benzocaine, acetylsalicylic acid, ascorbic acid and sucrose are tolerated at 10 −2 −10 −3 M levels. Hibitane 5% was analyzed successfully.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1986-01-01 | Analytica Chimica Acta |