6533b850fe1ef96bd12a840f
RESEARCH PRODUCT
Total RNA-isolation of abdominal hernia of rats for quantitative real-time reverse transcription (RT) PCR assays.
Domonkos FeherChristian MorsczeckJ.m. SchierholzMichael KorenkovManfred Nagelschmidtsubject
Incisional herniaAbdominal HerniaBiologyBiochemistryCollagen Type ITransforming Growth Factor beta1Gene expressionmedicineAnimalsHerniaGeneBase SequenceReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingGeneral Medicinemedicine.diseaseMolecular biologyReverse transcriptaseHernia AbdominalRatssurgical procedures operativeReal-time polymerase chain reactionCollagen Type IIIRNABiological AssayRNA extractionBiotechnologydescription
Abstract Increasing complications in incisional hernia surgery call for novel treatments. A gene expression analysis of injured tissues displays important parameters for tissue regeneration. Until today, no reliable method has been described for a quantitative gene expression analysis of hernia tissues. In this work, a protocol is described for the isolation of DNA‐free total RNA of incisional hernias for the first time. Moreover, real‐time RT PCR assays for collagen type I and III and TGF‐β1 are demonstrated for relative gene expression analyses. Both methods enable relative gene expression analyses of hernia tissues for the first time.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-12-01 | Preparative biochemistrybiotechnology |