6533b851fe1ef96bd12a8f77

RESEARCH PRODUCT

Biocompatibility and biodistribution of functionalized carbon nano-onions (f-CNOs) in a vertebrate model

Silvia GiordaniJuergen BartelmessRosaria BresciaMarina RodioFrancesca Cella ZanacchiMarta D’amoraGiuseppe SancataldoGiuseppe SancataldoAlberto DiasproAlberto Diaspro

subject

Biodistributionanimal structuresMultidisciplinarybiologyBiocompatibilityChemistryfungi02 engineering and technologyAnatomy010402 general chemistry021001 nanoscience & nanotechnologybiology.organism_classification01 natural sciencesArticle0104 chemical scienceschemistry.chemical_compoundIn vivoNano-ToxicityBiophysicsBODIPY0210 nano-technologyCytotoxicityZebrafish

description

AbstractFunctionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5–100 μg mL−1) and a homogenous biodistribution in zebrafish larvae.

yearjournalcountryeditionlanguage
2016-09-01
10.1038/srep33923http://hdl.handle.net/2318/1658335