6533b851fe1ef96bd12a8f99
RESEARCH PRODUCT
Protein differences among the Mediterranean species of the genus Spicara.
Matteo CammarataA MauroG. ScelsaMarco ArculeoParrinello NS. Lo Bruttosubject
Mediterranean climateSpicaragenetic distanceEcologyProtein electrophoresis; species differentiation; genetic distance; Spicara; Mediterranean Sea.species differentiationSettore BIO/05 - ZoologiaZoologyAquatic ScienceBiologyGel electrophoresis of proteinsbiology.organism_classificationGenetic distanceGenusGenetic markerBlack seaMediterranean Sea.SpicaraProtein electrophoresiEcology Evolution Behavior and Systematicsdescription
Protein electrophoresis (PAGE) was used to study the three morphologically different species of Spicara (S. flexuosa, S. maena, S. smaris). Of the 28 enzymatic and additional myogenic loci, five monomorphic loci (LDH-1*, G6PD-1*, PGI-1* and two PMMs*) were species-specific markers of S. smaris with respect to S. flexuosa and S. maena. Four of the 28 enzymatic loci were polymorphic (EST-1*, GLDH*, PEPD*, PGI-2*). Discriminating genetic markers were not identified between S. flexuosa and S. maena. Genetic distance (D) as calculated by Nei’s index (1978), between S. smaris v. S. maena and S. flexuosa showed a value, respectively of D=0·137 and 0·141. Between S. flexuosa and S. maena the value was D=0·006. From the data it can be inferred that S. flexuosa and S. maena are conspecific, despite morphological differences.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-12-01 |