6533b851fe1ef96bd12a90a0
RESEARCH PRODUCT
Quantitation of antihistamines in pharmaceutical preparations by liquid chromatography with a micellar mobile phase of sodium dodecyl sulfate and pentanol
Mayte Gil-agustíMaría Celia García-alvarez-coqueJosep Esteve-romeroLlorenç Monferrer-ponssubject
MicelleDosage formAnalytical Chemistrychemistry.chemical_compoundPentanolsmedicineCyclizineHumansEnvironmental ChemistrySodium dodecyl sulfateMicellesDosage FormsPharmacologyChromatographyChemistrySodium Dodecyl SulfatePharmaceutical PreparationsDoxylamineMicellar solutionsHistamine H1 AntagonistsCarbinoxamineAzatadineAgronomy and Crop ScienceChromatography LiquidFood Sciencemedicine.drugdescription
Abstract A reversed-phase liquid chromatographic procedure with a micellar mobile phase of sodium dodecyl sulfate (SDS), containing a small amount of pentanol, was developed for the control of 7 antihistamines of diverse action in pharmaceutical preparations (tablets, capsules, powders, solutions, and syrups): azatadine, carbinoxamine, cyclizine, cyproheptadine, diphenhydramine, doxylamine, and tripelennamine. The retention times of the drugs were <9 min with a mobile phase of 0.15M SDS–6% (v/v) pentanol. The recoveries with respect to the declared compositions were in the range of 93–110%, and the intra- and interday repeatabilities and interday reproducibility were <1.2%. The results were similar to those obtained with a conventional 60 + 40 (v/v) methanol–water mixture, with the advantage of reduced toxicity, flammability, environmental impact, and cost of the micellar-pentanol solutions. The lower risk of evaporation of the organic solvent dissolved in the micellar solutions also increased the stability of the mobile phase.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-01-05 |